Background
Halogenated nucleotides such because of the pyrimidine analog bromodeoxyuridine (BrdU) are helpful for labeling nascent DNA in residing cells and tissues. BrdU turns into built-in into replicating DNA in its place of thymidine and subsequent immunodetection of BrdU utilizing particular monoclonal antibodies permits labeling of cells in S part of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb may very well be utilized to detect BrdU built-in into single stranded DNA.
XTT Cell Proliferation Assays
In distinction to MTT, the cleavage product of XTT is soluble in water; a solubilization step is subsequently not required. The tetrazolium salt XTT is cleaved to formazan by a elaborate mobile mechanism. This bioreduction happens in viable cells solely, and is expounded to NAD(P)H manufacturing by means of glycolysis. The quantity of formazan dye frequent straight correlates to the variety of metabolically energetic cells contained in the customized.
WST-1 Cell Proliferation Assays
The common tetrazolium salt WST-1 is cleaved to a soluble formazan by a elaborate mobile mechanism that happens completely on the cell flooring. This bioreduction is principally counting on the glycolytic manufacturing of NAD(P)H in viable cells. The quantity of formazan dye frequent straight correlates to the variety of metabolically energetic cells contained in the customized.
Product Description
The BrdU Cell Proliferation Assay Package deal deal detects 5-bromo-2’-deoxyuridine (BrdU) built-in into mobile DNA all by cell proliferation utilizing an anti-BrdU antibody. When cells are cultured with labeling medium that comes with BrdU, this pyrimidine analog is built-in in its place of thymidine into the newly synthesized DNA of proliferating cells.
After eradicating labeling medium, cells are fastened and the DNA is denatured with our fixing/denaturing reply. Then a BrdU mouse mAb is added to detect the built-in BrdU (The denaturing of DNA is crucial to bolster the accessibility of the built-in BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to acknowledge the positive detection antibody. HRP substrate TMB is added to develop coloration. The magnitude of the absorbance for the developed coloration is proportional to the amount of BrdU built-in into cells, which is a direct indication of cell proliferation.
| Product Consists of | Amount (with Rely) | Reply Shade | |||
|---|---|---|---|---|---|
| BrdU | 1 x 150 µl | ||||
| Fixing/denaturing Reply | 2 x 25 ml | ||||
| BrdU Mouse Detection mAb | 1 x 500 µl | Inexperienced | |||
| Anti-mouse IgG, HRP-Linked Antibody | 1 x 500 µl | Pink | |||
| Detection Antibody Diluent | 1 x 50 ml | Inexperienced | |||
| HRP Diluent | 1 x 50 ml | Pink | |||
| 20X Wash Buffer | 1 x 50 ml | ||||
| TMB Substrate 7004 | 1 x 50 ml | ||||
| STOP Reply 7002 | 2 x 25 ml |
Key decisions and particulars
- Sensitivity: 40 cells/efficiently
- Pattern selection: Adherent cells, Suspension cells
- Detection method: Colorimetric
- Assay selection: Sandwich (qualitative)
5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It’ll get built-in into the newly synthesized DNA of proliferating cells in its place of thymidine. Biovision’s BrdU Cell Proliferation Assay Package deal deal detects built-in BrdU utilizing a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody positive to BrdU, which is adopted by addition of TMB (a HRP substrate). The extent of coloration enchancment is proportional to the amount of BrdU built-in into the cells and may very well be utilized straight as an indicator of cell proliferation. In contrast with utterly completely different cell proliferation assays, this bundle deal detects solely the proliferating cells and in no way the seeded cells. This terribly delicate, non-radioactive bundle deal detects as quite a bit a lot much less as 50-100 proliferating cells.
METABOLIC PROLIFERATION ASSAYS
Assays that measure metabolic prepare are acceptable for analyzing proliferation, viability, and cytotoxicity. The low price of tetrazolium salts similar to MTT, XTT, and WST-1 to coloured formazan compounds or the bioreduction of resazurin happens solely in metabolically energetic cells. Actively proliferating cells enhance their metabolic prepare, whereas cells uncovered to toxins might need decreased prepare.
MTT Cell Proliferation Assays
MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide; thiazolyl blue) is a water soluble tetrazolium salt yielding a yellowish reply when ready in media or salt decisions missing phenol pink. Dissolved MTT is reworked to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water-insoluble formazan is likely to be solubilized utilizing isopropanol or utterly completely different solvents, and the dissolved provides is measured spectrophotometrically utilizing absorbance as a perform of focus of reworked dye.
CytoSelect BrdU Competitive ELISA Kit |
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| CBA-5098 | Cell Biolabs | 96 assays | EUR 651.6 |
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNC942888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF594 conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNC942888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF594 conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNCA2888-250 | Biotium | 250uL | EUR 472.8 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), APC conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNC552888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF555 conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC552888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF555 conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNC612888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF660R conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC612888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF660R conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNCP2888-250 | Biotium | 250uL | EUR 472.8 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), PerCP conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNCR2888-250 | Biotium | 250uL | EUR 472.8 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), RPE conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNC402888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF640R conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC402888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF640R conjugate, Concentration: 0.1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC432888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF543 conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC432888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF543 conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNC052888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405M conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC052888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405M conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC812888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680R conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC812888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680R conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC802888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680 conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC802888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680 conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNCH2888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNCH2888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNUB2888-100 | Biotium | 100uL | EUR 316.8 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Concentration: 0.2mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
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| BNUB2888-50 | Biotium | 50uL | EUR 486 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), 1mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNUB2888-500 | Biotium | 500uL | EUR 615.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Concentration: 0.2mg/mL |
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Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC042888-100 | Biotium | 100uL | EUR 279.6 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405S conjugate, Concentration: 0.1mg/mL |
|||
Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody |
|||
| BNC042888-500 | Biotium | 500uL | EUR 654 |
|
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405S conjugate, Concentration: 0.1mg/mL |
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